| Congresso Brasileiro de Microbiologia 2023 | Resumo: 348-2 | ||||
Resumo:The increase of antimicrobial resistance to antibiotics has limited the treatments against infections caused by multidrug-resistant microorganisms. Natural products historically have been used as an alternative for the treatment of several diseases, included bacterial infections. In fact, plant species, such as the genus Croton, are widely known for their antimicrobial properties. Thus, the aim of this work was to extract and identify the chemical composition of the essential oil from Croton pluriglandulosus Carn. -Torres & Riina (OCp) leaves and evaluate its antibacterial activity. The plant material was collected in Serra do Lima in Patu-RN, and the extraction of essential oil was evaluated by hydrodistillation in a Clevenger apparatus. The chemical composition was identified by Gas chromatography-mass spectrometry (GC-MS). The antimicrobial activity of OCp was performed by Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration (MBC) assays against different bacterial strains, included Staphylococcus aureus ATCC 25923; S. aureus ATCC 700698; S. aureus JKD 6008; S. epidermidis 12228; S. epidermidis 35984; Pseudomonas aeruginosa ATCC 10145 and Eschericha coli ATCC 11303. OCp was diluted in microplates containing MHB medium with 4% DMSO at concentrations from 10 to 0.15 mg/ml-1, and the plates were incubated with each bacterial suspension (1 × 105 CFU/ml) for 24h at 37°C. Seventeen compounds, corresponding to 97.85% of the total oil, were identify by GC-MS. The majority constituents of OCp include α-pinene (14.26%), eucalyptol (25.63%) and 1H-Cycloprop[e]azulen-7-ol, decahydro-1,1,7 (14.77%). The OCp showed bacteriostatic and bactericidal effect against S. aureus strains. The MIC values were of 10 mg/ml-1 against S. aureus ATCC 25923, S. aureus ATCC 700698 and of 5 mg/ml-1 against S. aureus JKD 6008. Similar values were observed for MBC values. The major components of OCp show antibacterial activity reported in the literature, among them are α-Pinene and eucalyptol. The essential oils of Croton species show a wide variety of compounds with antimicrobial activities, but the inhibition of Gram-negative bacteria is little observed, possibly due to the bacterial structure that hinders the activity of the compounds. Furthermore, most EOs act directly on the bacterial membrane, causing lesion/deformation processes in their structures. In conclusion, OCp demonstrated activity against all three S. aureus strains tested, including methicillin-resistant S. aureus ATCC 700698 (MRSA) and vancomycin intermediate resistant S. aureus JKD 6008 (VISA). The antimicrobial activity possibly occurs due to the major compounds present in the oil. Thus, OCp presents itself as a potential compound for the treatment of infections caused by S. aureus. Palavras-chave: antibacterial activity, Croton, essential oil, medicinal plants Agência de fomento:Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq), Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES), Fundação de Amparo à Pesquisa do Estado de Minas Gerais (FAPEMIG). | |||||